CALLISTEPHIN CHLORIDE WITH HPLC
Pelargonidin 3-glucoside chloride
CAS: 18466-51-8
Molecular Formula: C21H21ClO10
CALLISTEPHIN CHLORIDE WITH HPLC - Names and Identifiers
CALLISTEPHIN CHLORIDE WITH HPLC - Physico-chemical Properties
| Molecular Formula | C21H21ClO10 |
| Molar Mass | 468.84 |
| Storage Condition | -20°C |
| MDL | MFCD00017578 |
CALLISTEPHIN CHLORIDE WITH HPLC - Risk and Safety
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-21 |
CALLISTEPHIN CHLORIDE WITH HPLC - Reference
| Reference Show more | 1. Ma Jin, Liu Lu, Liu Guoying, et al. Simultaneous determination of five anthocyanins in Mulberry health wine by UPLC-Q-Tof/MS [J]. Wine 2019(4):98-100. 2. Li, Wenfeng, et al. "Degradation of pelargonidin-3-(p-coumaroyl) diglucoside-5-(malonyl) glucoside and pelargonidin-3-(ferroyl) diglucoside-5-(malonyl) glucoside in red radiation during air-impingement jet drying." LWT 127 (2020): 109390.https:// doi 3. Yang, Jing, et al. "Evaluation of physichemical properties in three (Rubus idaeus) at five ripening stages in northern China." Scientia Horticulturae 263 (2020): 109146.https://doi.org/10.1016/j.scienta.2019.109146 4. [IF=4.952] Wenfeng Li et al."Glycosides changed the stability and antioxidant activity of pelargonidin."Lwt Food Sci Technol. 2021 Jul;147:111581 5. [IF=4.952] Wenfeng Li et al."Degradation kinetics of pelargonidin-3-(p-coumaroyl)diglucoside-5-(malonyl)glucoside and pelargonidin-3-(feruloyl)diglucoside-5-(malonyl)glucoside in red radish during air-impingement jet drying."Lwt Food Sci Technol. 2020 Jun;127:109390 6. [IF=4.952] Yuxin Hao et al."Stability and mechanism of phenolic compounds from raspberry extract under in vitro gastrointestinal digestion."Lwt Food Sci Technol. 2021 Mar;139:110552 7. [IF=4.192] Suwen Liu et al."Cerasus humilis Cherry Polyphenol Reduces High-Fat Diet-Induced Obesity in C57BL/6 Mice by Mitigating Fat Deposition, Inflammation, and Oxidation."J Agr Food Chem. 2020;68(15):4424-4436 8. [IF=3.463] Jing Yang et al."Evaluation of physicochemical properties in three raspberries (Rubus idaeus) at five ripening stages in northern China."Sci Hortic-Amsterdam. 2020 Mar;263:109146 9. [IF=6.793] Luo Xiaoning et al.Integrating full-length transcriptomics and metabolomics reveals the regulatory mechanisms underlying yellow pigmentation in tree peony (Paeonia suffruticosa Andr.) flowers.Hortic Res-England. 2021 Nov;8(1):1-15 10. [IF=5.396] Xinyue Zhang et al."Blue honeysuckle extracts retarded starch digestion by inhibiting glycosidases and changing the starch structure."Food & Function. 2022 May ;: |
CALLISTEPHIN CHLORIDE WITH HPLC - Uses and synthesis methods
Overview
Geranium-3-O-glucoside (Pelargonidin3-O-glucoside, hereinafter referred to as Pg3G) is the most representative compound in anthocyanins, and its water solubility is good. At present, Pg3G has been found in fruits and vegetables such as strawberry, red raspberry, red radish, mangosteen skin and black bean. Among them, strawberry anthocyanin is mainly Pg3G, and its content accounts for 80-95% of strawberry anthocyanin. It is currently an ideal raw material for extracting Pg3G. Recent studies have shown that Pg3G has antioxidant and oxidative stress protection effects. Therefore, food-derived Pg3G can be used not only as a natural pigment for food processing, but also as a natural antioxidant and functional food factor for the development of health food. Geranium -3-glucoside chloride is the chloride of geranium -3-O-glucoside.
Preparation
The kaempferol -3-(6-O-acetyl) glucoside (9.75mg,0.020mmol) and Zn powder (164mg) were dried under vacuum and contained in the reactor. Subsequently, Ar gas was introduced into the reactor and the inside of the reactor was made into an Ar atmosphere. At 0 ℃, 3.5% hydrogen chloride-anhydrous methanol solution (1.2ml) was added under ultrasonic irradiation. Ultrasonic irradiation and strong stirring were carried out for 7 minutes, and then the reaction mixture was filtered under dry conditions to remove Zn powder to obtain filtrate. The above filtrate is contacted with dry air at room temperature, and vigorously stirred to react for 3.5 hours. The color of the reaction mixture changes from light red to dark red. Pour the reaction mixture into water (30ml). Pour it into a column (1.5cm inside diameter, 17cm high) filled with "AmberliteXAD-7 gel" (replaced by 0.5% TFA aqueous solution), and adsorb the dye molecules on the column. Subsequently, the column was washed with 0.5% aqueous solution of TFA (250ml). Then, 90% acetonitrile aqueous solution containing 0.5% TFA was added to elute the adsorbed dye in the column. Refined pigment fractions were ODS-HPLC by preparation separation. As a result of HPLC analysis using ODS columns, the pigment fraction contained two anthocyanins. The resulting pigment fraction was concentrated to dryness at 40°C under reduced pressure. 5.8mg of geranium glycoside -3-(6-O-acetyl) glucoside (yield 50%) and 5.4mg of geranium -3 glucoside (yield 50%) were obtained. Geranium -3 glucoside and hydrochloric acid salt preparation of geranium -3 glucoside chloride. The purity of the pigment fraction of any pigment shall not be less than 95%.
Last Update：2024-04-09 21:21:28
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